65035-M

Trypsin Inhibitor, Soybean

• Synonym(s): Trypsin Inhibitor, Soybean
• CAS Number: 9035-81-8
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• form: lyophilized solid
• Quality Level: 100
• specific activity: ≥4000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: off-white
• solubility: 67 mM potassium phosphate buffer, pH 7.6: 5 mg/mL
• storage temp.: −20°C

Description

General description

A reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, pH-dependent manner (K_d = 500 nM at pH 7.8).

Reversible inhibitor of trypsin that binds to trypsin in a stoichiometric, reversible and pH-dependent manner (K_d = 0.5 nM at pH 7.8). Also inhibits, to a lesser degree, chymotrypsin, kallikrein, plasmin, and other serine proteases.

Biochem/physiol Actions

Kd = 500 nM at pH 7.8 against trypsin

Primary Target
trypsin

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C.

Other Notes

Lilly, C.M., et al. 1993. J. Clin. Invest.91, 235.
Uchino, T., et al. 1993. J. Biol. Chem.268, 527.
Birk, Y. 1976. Methods Enyzmol.45, 700.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable