559309

PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem^®

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat
• Application: —
• Citations: 8

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: phosphorylation (pThr183/pTyr185)
• Gene Information: human ... MAPK8(5599)

Description

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr¹⁸³ and Tyr¹⁸⁵.

Antibody Target Gene Symbol: MAPK8, MAPK9

Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1

Entrez Gene Name: mitogen-activated protein kinase 8

Hu Entrez ID: 5601

Mu Entrez ID: 26420

Rat Entrez ID: 50658

This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK

Application

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 50% glycerol, 1 mg/ml BSA, pH 7.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
Gabai, V.L., et al. 1998. FEBS Lett.438, 1.

Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable