5.34360

Autophagy Inhibitor VII

• Synonym(s): Autophagy Inhibitor VII, 6-Chloro-N-(1-ethylpiperidin-4-yl)-1,2,3,4-tetrahydroacridin-9-amine, Acridinamine 33, Tetrahydroacridine 33, THA33
• Empirical Formula (Hill Notation): C₂₀H₂₆ClN₃
• Molecular Weight: 343.89
• NACRES: NA.77
• UNSPSC Code: 12352200

Properties

• assay: ≥98% (HPLC)
• Quality Segment: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• color: white to pale yellow
• solubility: DMSO: 1.6 mg/mL, water: 50 mg/mL
• storage temp.: −20°C

Description

General description

A cell-permeable tetrahydroacridinamine (THA) derived compound that potently suppresses autophagy (EC = 250 nM) with reduced toxicity (LD₅₀ = 27 µM) in U2OS cells. Shown to induce lysosome deacidification and increase LC3-II protein accumulation. Displays attractive biophysical properties.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Biochem/physiol Actions

Cell permeable: yes

Reversible: yes

Packaging

Packaged under inert gas

Physical form

Supplied as HCl salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Wang, T., et al. 2015. J. Med. Chem.58, 3025.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable