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36-006

Sigma-Aldrich

Anti-phospho-CPI-17 (Thr38) Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rabbit, bovine, human, pig, mouse, rat

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

isotype

IgG

NCBI accession no.

NM_033256.1

UniProt accession no.

Q96A00

shipped in

dry ice

target post-translational modification

phosphorylation (pThr38)

Gene Information

bovine ... Ppp1R14A(615348)
human ... PPP1R14A(94274)
mouse ... Ppp1R14A(68458)
pig ... Ppp1R14A(397610)
rat ... Ppp1R14A(114004)

Specificity

CPI-17 phosphorylated on Threonine 38.

Immunogen

Peptide corresponding to amino acids 35-46 of human CPI-17.

Application

Detect phospho-CPI-17 (Thr38) using this Anti-phospho-CPI-17 (Thr38) Antibody validated for use in EA.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunoblot on phosphorylated recombinant CPI-17.

Target description

~17 kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%.
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T Kitazawa et al.
The Journal of biological chemistry, 275(14), 9897-9900 (2000-04-01)
Myosin light chain phosphatase (MLCP) plays a pivotal role in smooth muscle contraction by regulating Ca(2+) sensitivity of myosin light chain phosphorylation. A smooth muscle phosphoprotein called CPI-17 specifically and potently inhibits MLCP in vitro and in situ and is
Increased PDE5 activity and decreased Rho kinase and PKC activities in colonic muscle from caveolin-1-/- mice impair the peristaltic reflex and propulsion.
Mahavadi, S; Bhattacharya, S; Kumar, DP; Clay, C; Ross, G; Akbarali, HI; Grider, JR; Murthy, KS
American Journal of Physiology: Gastrointestinal and Liver Physiology null
Ketrija Touw et al.
American journal of physiology. Gastrointestinal and liver physiology, 302(1), G66-G76 (2011-10-08)
Seventy-six percent of diabetic patients develop gastrointestinal symptoms, such as constipation. However, the direct effects of diabetes on intestinal smooth muscle are poorly described. This study aimed to identify the role played by smooth muscle in mediating diabetes-induced colonic dysmotility.
Solution NMR structure of the myosin phosphatase inhibitor protein CPI-17 shows phosphorylation-induced conformational changes responsible for activation.
Ohki, S, et al.
Journal of Molecular Biology, 314, 839-849 (2001)
Young Jae Im et al.
American journal of physiology. Renal physiology, 313(2), F370-F377 (2017-04-28)
This study was performed to analyze the developmental changes in bladder response to cholinergic stimulation in detail, highlighting calcium sensitization (CS) and its related pathways. Rats were divided into three groups in accordance with reported time of developmental milestones (newborns
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