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194932

AZOCOLL Substrate, <50 Mesh

General proteolytic substrate.

Synonym(s):

AZOCOLL Substrate, <50 Mesh

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Size/SKUAvailabilityPrice
1 g
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$176.00

About This Item

CAS Number:
MDL number:
NACRES:
NA.77
UNSPSC Code:
12352200
Form:
solid
Storage condition:
OK to freeze, protect from light

$176.00


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Quality Segment

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

dark red

solubility

aqueous buffer: soluble, water: soluble

shipped in

ambient

storage temp.

10-30°C

General description

General proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL Substrate with 2 units of PRONASE Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm.
This product has been discontinued.


General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%).

Other Notes

Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
19493327667344935
description

General proteolytic substrate.

description

General proteolytic substrate.

description

for use at 320 nm

description

A fluorogenic substrate for furin, a mammalian homolog of the yeast Kex2 endoprotease (kcat/Km ~ 2 x 10⁴ s⁻¹M⁻¹).

form

solid

form

solid

form

powder or crystals

form

solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

protect from light, OK to freeze

storage condition

OK to freeze, protect from light

storage condition

-

storage condition

desiccated (hygroscopic), OK to freeze, protect from light

solubility

water: soluble, aqueous buffer: soluble

solubility

PBS: 10 mg/mL

solubility

-

solubility

water: 10 mg/mL, DMSO: soluble


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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