16-220

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate

Upstate^®, from mouse

• Synonym(s): Anti-MPM-2 Cy5 Antibody, Cy5 Anti-MPM-2, Phospho-Ser/Thr-Pro Detection
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: CY5 conjugate
• Clone: monoclonal
• Application: ICC, IF, WB
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: CY5 conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer/pThr)

Description

General description

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.

Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Also, known as Forkhead-related protein FKHL16 or Hepatocyte nuclear factor 3 forkhead homolog 11.

Immunogen

Mitotic human HeLa cell lysate

Application

Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in IF, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Recognizes a phosphorylated epitope (phospho-[Ser/Thr]Pro) found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.

Physical form

100μg Cy5-conjugated mouse IgG₁ in 200μl of PBS containing 1% BSA, 0.05% Tween^®-20, 0.05% sodium azide.

Protein G Purified

Preparation Note

1 year at 4°C from date of shipment

Analysis Note

Control
Colcemid-treated HeLa cell lysates

Routinely evaluated by immunoblot on colcemid-treated HeLa cell lysates.

Legal Information

TWEEN is a registered trademark of Croda International PLC

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable