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1.50657

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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1 pkg

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$1,580.00

About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
3 μm
Matrix active group:
ZIC-HILIC, ZIC-cHILIC bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle

$1,580.00


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Product Name

SeQuant® ZIC®-cHILIC 3µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Segment

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC, ZIC-cHILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

1-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 100 mm length x 2.1 mm ID is the best choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
1.506621.506581.50659
particle size

3 μm

particle size

3 μm

particle size

3 μm

particle size

3 μm

matrix active group

ZIC-cHILIC bonding phase

matrix active group

ZIC-cHILIC bonding phase

matrix active group

ZIC-cHILIC bonding phase

matrix active group

ZIC-cHILIC bonding phase

separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

separation technique

hydrophilic interaction (HILIC)

product line

SeQuant®

product line

SeQuant®

product line

SeQuant®

product line

SeQuant®

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

pore size

100 Å pore size

pore size

100 Å pore size

pore size

100 Å pore size

pore size

100 Å pore size


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Protocols

HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

ZIC®-cHILIC is a densely bonded zwitterionic stationary phase with phosphorylcholine functional groups covalently attached to silica.

Articles

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

Ready-to-use Polar Metabolites QC Mix for detecting instrumental drift in the LC-MS metabolomics analysis workflow for both ESI(+) and ESI(−).

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Las moléculas pequeñas son iones y compuestos con un peso molecular inferior a 900 daltons. Estos compuestos pueden separarse y analizarse eficazmente mediante HPLC, UHPLC y LC-MS utilizando principalmente partículas de sílice o fases estacionarias monolíticas con una amplia gama de composiciones químicas de columna (modificaciones).

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

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Global Trade Item Number

SKUGTIN
150657000104027269863005

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