1.50438

SeQuant^® ZIC^®-pHILIC (5 µm) HPLC Columns

L x I.D. 20 mm x 2.1 mm guard Column Kit (3 pc) with coupler (1 pc)

• Synonym(s): Hydrophilic Interaction Liquid Chromatography Guard Column
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC
• Pore size: 100 Å pore size
• Matrix: polymeric particle platform

Properties

• Product Name: SeQuant^® ZIC^®-pHILIC Guard Kit 20 x 2.1 mm, PEEK-lined stainless steel column, pkg of 3 column
• material: PEEK-lined stainless steel column
• Quality Segment: 100
• agency: suitable for USP L122
• product line: SeQuant^®
• feature: endcapped: no
• packaging: pkg of 3 column
• parameter: 200 bar pressure, 50 °C max. temp.
• technique(s): LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 2 cm × 2.1 mm
• matrix: polymeric particle platform
• matrix active group: ZIC-HILIC, Zwitterion bonding phase, ZIC-pHILIC
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH: 2-10
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

The Guard column kit contains 3 SeQuant^® ZIC^®-pHILIC guard columns including the fitting to connect to the analytical column.SeQuant^® ZIC^®-pHILIC stationary phase has the same highly polar, bonded, permanent zwitterion, functional group as the silica based ZIC^®-HILIC Column. The user can therefore expect the same selectivity, however, with a trade-off in flow-rate range, and separation efficiency, common with polymeric materials. The more durable support allows for use in an extended pH range, which can be beneficial for certain applications.SeQuant^® ZIC^®-pHILIC HPLC columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Offering excellent pH stability from pH 2 to 10, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. This results in straightforward and reproducible separations with wide-spectrum pH tuning for optimal separation selectivity and detection sensitivity. Furthermore, SeQuant^® ZIC^®-pHILIC columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD). SeQuant^® ZIC^®-pHILIC columns are available with 5 μm polymer particles in various formats of analytical dimensions. Alternative choices for the separation of polar hydrophilic compounds include silica-based SeQuant^® ZIC^®-HILIC columns with identical functional groups, and SeQuant^® ZIC^®-cHILIC columns with complementary selectivity.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

performance test cytosine
Extent of testing: conforms

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany