The specific isoform is not identified. The SDS PAGE reveals a molecular weight of 47 kDa for this product. However, this molecular weight includes a C-terminal 6His tag (0.8 kDa) and an N-terminal GST tag (approx. 26 kDa). The molecular weight of the remaining recombinant protein is just over 20 kDa. See the link below to review the product datasheet, which includes the sequence:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/610/13-173.pdf
13-173
MBP, recombinant
Synonym(s):
myelin basic protein
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| Size/SKU | Availability | Price |
|---|---|---|
500 μg | Check Cart for Availability | $489.00 |
About This Item
UNSPSC Code:
12352200
NACRES:
NA.41
eCl@ss:
32160405
Form:
liquid
Biological source:
bovine
Mol wt:
Mw ~47 kDa
biological source
bovine
Quality Segment
form
liquid
mol wt
Mw ~47 kDa
manufacturer/tradename
Upstate®
concentration
1 mg/mL
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Physical form
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
ProBond Resin chromatography
Preparation Note
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Routinely evaluated by kinase assay
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
Other Notes
Purity information is lot specific, please consult the certificate of analysis
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| biological source bovine | biological source human | biological source mouse | biological source mouse |
| technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) ELISA: suitable, electron microscopy: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable |
| concentration 1 mg/mL | concentration 0.5 mg/mL | concentration - | concentration - |
| mol wt Mw ~47 kDa | mol wt Mw 72 kDa | mol wt - | mol wt - |
| form liquid | form liquid | form - | form - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which MBP isoform is product 13-173 (SKU)? Can I please have the sequence of it?
1 answer-
Helpful?
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