09-823

Anti-JMJD1A Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): JMJD, lysine (K)-specific demethylase 3A, testis-specific protein A, jumonji domain containing 1A, jumonji C domain-containing histone demethylase 2A, jumonji domain containing 1, Jumonji domain-containing protein 1A, JmjC domain-containing histone demet
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, IP, WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_001033784.2, NP_766589.1
• UniProt accession no.: Q6PCM1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KDM3A(55818)

Description

General description

JMJD1A (jumonji domain-containing 1a) is a histone demethylase that plays a key role in the histone code. Functionally, this protein is known to demethylate mono- and dimethylated histoneH3 at lysine 9, while demonstrating virtually no demethylation activity for trimethylated lysine 9 of histone H3. A product of the JHDM2A gene, this protein has been demonstrated to play a role in hypoxia, spermatogenesis, and cell differentiation with loss of function of JMJD1A resulting embryonic stem cell differentiation.

~147 kDa observed.

Immunogen

GST-tagged recombinant protein corresponding to mouse JMJD1A.

Application

Immunoprecipitation Analysis: 5 µg of a representative lot of this antibody immunoprecipitated JMJD1A in HeLa nuclear extract.

Western Blot Analysis: A representitive lot of this antibody detected JMJD1A in mouse ESC chromatin extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-JMJD1A Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 nuclear extract.

Evaluated by Western Blot in HEK293 nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected JMJD1A on 10 µg of HEK293 nuclear extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable