09-774

Anti-EED Antibody

from rabbit

• Synonym(s): embryonic ectoderm development
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 42

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse
• species reactivity (predicted by homology): dog, chicken, bovine, opossum, rat
• technique(s): western blot: suitable
• NCBI accession no.: NP_003788
• UniProt accession no.: O75530
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: dog ... Eed(476779) ; human ... EED(8726) ; mouse ... Eed(13626) ; rat ... Eed(293104)

Description

General description

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

~53 kDa observed. Uniprot describes three isoforms at 51 kDa (isoform 1), 53 kDa (isoform 2), and 46 kDa (isoform 3).

Immunogen

A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH.

Epitope: a.a. 429-441

Application

Use Anti-EED Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EED also known as embryonic ectoderm development.

Biochem/physiol Actions

This antibody recognizes EED, Mr ~ 62-70 kDa.

Physical form

Format: Purified

Analysis Note

Control
Jurkat Cell Lysate

3T3/A31 cell lysate

Western Blot Analysis: 1 μg/mL of this lot detected EED on 10 μg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 09-727

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable