07-1386

Anti-Connective Tissue Growth Factor Antibody

from goat

• Synonym(s): Hypertrophic chondrocyte-specific protein 24, connective tissue growth factor, insulin-like growth factor-binding protein 8
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: -

Properties

• biological source: goat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_001892.1
• UniProt accession no.: P29279
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CTGF(1490)

Description

General description

Connective Tissue Growth Factor (CTGF) is a member of the CNN family of growth factors that are characterized as having cysteine-rich peptides with four specific domains. CTGF was originally identified in endothelial cells as a significant chemotactic and mitogenic factor. Research has revealed CTGF to have several biological properties which include chemotaxis, cell proliferation, promotion of adhesion and the formation of extracellular matrix. It is also necessary for such biological processes as wound healing, scar formation, fibrosis, embryogenesis and angiogenesis. In its role as an inducer of angiogenesis, CTGF has been associated with vascular endothelial growth factor (VEGF). As a result of its pro-fibrotic properties, CTGF is thought to be a promising target for therapy in conditions involving pathological fibrosis such as chronic fibrotic disease, chronic kidney disease and proliferative diabetic retinopathy (PDR). Cell types found to express CTGF are endothelial cells, fibroblasts and vascular smooth-muscle cells.

In Western Blot, full length CTGF is observed at 38 kDa and CTGF N&C domain is observed at 19 kDa

Immunogen

Epitope: Mature chain

Recombinant protein corresponding to the mature chain of human Connective tissue growth factor.

Application

Anti-Connective Tissue Growth Factor Antibody detects level of Connective Tissue Growth Factor & has been published & validated for use in WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Primate (98% sequence homolgy). Monkey (97% sequence homology). Porcine and Bovine (93% sequence homology). Rat and mouse (91% sequence homology).

This antibody recognizes the mature chain of Connective tissue growth factor.

Physical form

Format: Purified

Protein G Purified

Purified goat polyclonal in buffer containing Tris-Glycine pH 8.0 with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Untreated and TGFb1 treated NRK cell lysates

Evaluated by Western Blot in untreated and TGFb1 treated NRK cell lysates.

Western Blot Analysis: 5 µg/mL of this antibody detected Connective tissue growth factor on 10 µg of untreated and TGFb1 treated NRK cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable