790326C

Fluorescent Transfection Reagent

Avanti Research^™ - A Croda Brand 790326C

• Synonym(s): Fluorescent Transfection Reagent, Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• form: liquid
• packaging: pkg of 1 × 1 mL (790326C-10mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand 790326C
• concentration: 10 mg/mL (790326C-10mg)
• application(s): advanced drug delivery
• lipid type: cationic lipids; transfection
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fluorescent Transfection Reagent has the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine in the ratio 1:1 w/w along with 0.1 wt% N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) DOPE. NBD-DOPE is a fluorescence label. NBD is attached to headgroup of DOPE.

Application

Fluorescent Transfection Reagent has been used to visualize the localization of liposome oligodeoxynucleotides (ODN) complex in lung alveolar cells.

Biochem/physiol Actions

Fluorescent Transfection Reagent is useful in understanding fibroplex membrane structure and interaction of fibroin particles with cationic lipids. N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) is used in model membranes studies and is useful in visualizing membrane domains, at 460/535 nm (excitation/emission) wavelengths. The DOTAP:DOPE with 0.1% NBD-DOPE liposome (LipNBD) reconstituted with transferrin called the transferrin liposome-nitrobenzoxadiazole (Tf-LipNBD) is useful as a probe. It provides improved image contrast in in vitro magnetic resonance imaging (MRI) of tumor cells.

Packaging

5 mL Clear Glass Sealed Ampule (790326C-10mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302,H315,H319,H331,H336,H351,H361d,H372
• pcodes: P201 - P260 - P264 - P280 - P304 + P340 + P311 - P403 + P233
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: does not flash
• flash_point_c: does not flash