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L040031

Sigma-Aldrich

Cap A

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About This Item

UNSPSC Code:
12352200

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

concentration

97.0-119.0 g/L (acetic anhydride, titration)

color

clear colorlessto brownish

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General description

Cap A solution consists of acetic anhydrideanhydride, lutidine, and tetrahydrofuran (THF).

Application

Cap A solution is used after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

supp_hazards

EUH019

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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