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HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide

HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

565324-U

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47783

(±)-α-Tocopherol

analytical standard

V-025

Vitamin D3 solution

1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

acetonitrile

flow rate

2.0 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

20 μL

Description

Analysis Note

The Ascentis RP-Amide is shown to be suitable for the analysis of fat soluble vitamins, alpha tocopherol (vitamin E) and cholecalciferol (vitamin D3 ).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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