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  • Quantification of Bacteria Residing in Caenorhabditis elegans Intestine.

Quantification of Bacteria Residing in Caenorhabditis elegans Intestine.

Bio-protocol (2021-03-05)
M Fernanda Palominos, Andrea Calixto
ABSTRACT

Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes induced by bacterial pathogens using fluorescently-tagged bacteria. Furthermore, these methods can be used to isolate and identify new culturable bacterial species from natural microbiomes of wild nematodes.

MATERIALS
Product Number
Brand
Product Description

USP
Levamisole hydrochloride, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Ampicillin, anhydrous, 96.0-102.0% (anhydrous basis)
Sigma-Aldrich
Glycerol, ≥99.5%
Sigma-Aldrich
Gentamicin sulfate, meets EP, USP testing specifications
Sigma-Aldrich
Calcium chloride dihydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%