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63630

Millipore

D-(+)-Melibiose

suitable for microbiology, ≥99.0%

Synonym(s):

Raffinose, Melizitose, α-D-Galactosyl-(1→6)-α-D-glucopyranoside, Galactosyl D-glucose, Galactosylglucose, D-Galactopyranosyl-(1→6)-D-glucose, Melibiose, Galactinol, D-(+)-Galactosyl-(1→6)-D-(+)-glucose, α-D-Gal(1→6)β-D-Glc, 6-O-α-D-Galactopyranosyl-D-glucose

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About This Item

Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
Molecular Weight:
342.30
Beilstein:
93360
EC Number:
MDL number:
UNSPSC Code:
41106212
PubChem Substance ID:
NACRES:
NA.74

Quality Level

Assay

≥99.0% (HPLC)
≥99.0%

form

powder

optical activity

[α]20/D +137±3°, 10 hr, c = 5% in H2O

packaging

pkg of 10 g
pkg of 50 g

ign. residue

≤0.1% (as SO4)

color

colorless to white

solubility

H2O: 0.1 g/mL, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤200 mg/kg

cation traces

As: ≤0.1 mg/kg
Ca: ≤500 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤10 mg/kg
K: ≤50 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

microbiology

SMILES string

OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H22O11/c13-1-4(15)7(17)8(18)5(16)3-22-12-11(21)10(20)9(19)6(2-14)23-12/h1,4-12,14-21H,2-3H2/t4-,5+,6+,7+,8+,9-,10-,11+,12-/m0/s1

InChI key

AYRXSINWFIIFAE-GFRRCQKTSA-N

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General description

D-(+)-Melibiose is a reducing disaccharide composed of a unit of galactose linked to a unit of glucose by a glycosidic bond α (1 → 6). It is commonly found in plants and the roots of certain legumes. D-(+)-Melibiose is not metabolized by humans but serves as a substrate for certain bacteria and fungi. It is used as a differential medium component in microbiological media to detect the fermentation of melibiose by microorganisms. It helps identify specific bacterial species based on their ability to ferment melibiose.

Application

D-(+)-Melibiose has applications in the fields of microbiology, research and development, and in food and pharmaceutical industries. Melibiose fermentation test is used to differentiate bacteria like Enterobacteriaceae (like Salmonella, E.coli, shigella, Citrobacter, Klebsiella, Enterobacter) and yeasts. It is also used for the differentiation between the plant pathogenic bacteria Klebsiella pneumoniae and K. variicola.

Storage and Stability

Keep the container tightly closed in a dry and well-ventilated place.Hygroscopic, Store under inert gas.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nicole Hugouvieux-Cotte-Pattat et al.
Journal of bacteriology, 191(22), 6960-6967 (2009-09-08)
Erwinia chrysanthemi (Dickeya dadantii) is a plant pathogenic bacterium that has a large capacity to degrade the plant cell wall polysaccharides. The present study reports the metabolic pathways used by E. chrysanthemi to assimilate the oligosaccharides sucrose and raffinose, which
Ville P Heljo et al.
Journal of pharmaceutical sciences, 102(1), 195-203 (2012-11-15)
Melibiose monohydrate has shown promise when employed as a pharmaceutical excipient, but its physical properties have not been adequately characterized. Therefore, two different melibiose monohydrate batches were analyzed as received or after storage under different relative humidity (RH) atmospheres. The
Lan Guan et al.
The Journal of biological chemistry, 286(8), 6367-6374 (2010-12-15)
The MelB permease of Salmonella typhimurium (MelB-ST) catalyzes the coupled symport of melibiose and Na(+), Li(+), or H(+). In right-side-out membrane vesicles, melibiose efflux is inhibited by an inwardly directed gradient of Na(+) or Li(+) and stimulated by equimolar concentrations
Meritxell Granell et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(51), 22078-22083 (2010-12-08)
The melibiose carrier from Escherichia coli (MelB) couples the accumulation of the disaccharide melibiose to the downhill entry of H(+), Na(+), or Li(+). In this work, substrate-induced FTIR difference spectroscopy was used in combination with fluorescence spectroscopy to quantitatively compare
Peng Lin et al.
Acta biochimica et biophysica Sinica, 40(12), 1029-1038 (2008-12-18)
A dimeric 50 kDa melibiose-binding lectin was isolated from the seeds of the cultivar of soybean (Glycine max), called the small glossy black soybean. The isolation procedure comprised ion exchange chromatography on Q Sepharose, SP Sepharose and Mono Q followed

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