TAQN-RO
Roche
Taq DNA Polymerase, dNTPack
suitable for PCR, optimum pH ~9.0 (20 °C), dNTPs included
Synonym(s):
dna amplification, pcr, polymerase, primer extension
About This Item
biological source
bacterial (Thermus aquaticus BM)
Quality Level
recombinant
expressed in E. coli
description
5 U/βl
Number of Reactions:
If 1.25 U are used per 50 μL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage.
form
liquid
usage
sufficient for 2,000 reactions (04728904001)
sufficient for 4,000 reactions (04728858001)
sufficient for 400 reactions (04728874001)
sufficient for 80 reactions (04728866001)
sufficient for 800 reactions (04728882001)
mol wt
95 kDa
feature
dNTPs included
hotstart: no
packaging
pkg of 1,000 U (04728882001 [4 x 250 U])
pkg of 2,500 U (04728904001 [10 x 250 U])
pkg of 5,000 U (04728858001 [20 x 250 U])
pkg of 100 U (04728866001)
pkg of 500 U (04728874001 [2 x 250 U])
manufacturer/tradename
Roche
concentration
0.025 units/reaction
parameter
72 °C optimum reaction temp.
technique(s)
PCR: suitable
color
colorless
input
purified DNA
optimum pH
~9.0 (20 °C)
solubility
water: soluble
suitability
suitable for PCR and automated sequencing reactions
UniProt accession no.
application(s)
genomic analysis
life science and biopharma
foreign activity
endonucleases, none detected
exonuclease, none detected
nicking activitities, none detected
storage temp.
−20°C
Related Categories
General description
Taq DNA Polymerase, 5 U/μl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Application
- PCR
- RT-PCR
- Other primer-extension reactions, such as sequencing and labeling
Features and Benefits
- Reliable reproducible results: High lot-to-lot consistency.
- No need to test each lot: Taq DNA Polymerase is rigorously tested.
- Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.
Packaging
Quality
Unit Definition
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/μl
Storage and Stability
kept upright to prevent leakage
Other Notes
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
Legal Information
Kit Components Only
- Taq DNA Polymerase 5 U/μl
- PCR Buffer with MgCl<sub>2</sub> 10x concentrated
- PCR Nucleotide Mix
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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