Skip to Content
Merck
All Photos(1)

Key Documents

559309

Sigma-Aldrich

PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

liquid, Calbiochem®

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

phosphorylation (pThr183/pTyr185)

Gene Information

human ... MAPK8(5599)

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
  • Antibody Target Gene Symbol: MAPK8, MAPK9
  • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
  • Entrez Gene Name: mitogen-activated protein kinase 8
  • Hu Entrez ID: 5601
  • Mu Entrez ID: 26420
  • Rat Entrez ID: 50658
  • This PhosphoDetect Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

    Immunogen

    Human
    a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK

    Application

    Immunoblotting (1:1000)

    Immunohistochemistry (see comments)

    Warning

    Toxicity: Standard Handling (A)

    Physical form

    In Dulbecco′s PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.

    Reconstitution

    Following initial thaw, aliquot and freeze (-70°C).

    Other Notes

    Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
    Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
    Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    Not finding the right product?  

    Try our Product Selector Tool.

    Storage Class Code

    12 - Non Combustible Liquids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable


    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Sitong Wang et al.
    Frontiers in pharmacology, 13, 922457-922457 (2022-07-30)
    Background: Tumours are among the most lethal diseases that heavily endanger human health globally. Xuefu Zhuyu Decoction (XFZYD) is a prescription used to treat blood-activating stasis. Although XFZYD has been shown to suppress migration and invasion of tumour cells, the
    Chenxi Wu et al.
    Cell death discovery, 7(1), 202-202 (2021-08-06)
    Cancer is one of the most fatal diseases that threaten human health, whereas more than 90% mortality of cancer patients is caused by tumor metastasis, rather than the growth of primary tumors. Thus, how to effectively control or even reverse
    Ashutosh Pandey et al.
    Nature communications, 14(1), 5667-5667 (2023-09-14)
    Intestinal barrier dysfunction leads to inflammation and associated metabolic changes. However, the relative impact of gut bacteria versus non-bacterial insults on animal health in the context of barrier dysfunction is not well understood. Here, we establish that loss of Drosophila
    Chenxi Wu et al.
    Cell death & disease, 10(12), 893-893 (2019-11-28)
    Cell death plays a pivotal role in animal development and tissue homeostasis. Dysregulation of this process is associated with a wide variety of human diseases, including developmental and immunological disorders, neurodegenerative diseases and tumors. While the fundamental role of JNK
    Ihab H Hassan et al.
    The Journal of biological chemistry, 287(7), 4679-4689 (2011-12-24)
    Known therapies for influenza A virus infection are complicated by the frequent emergence of resistance. A therapeutic strategy that may escape viral resistance is targeting host cellular mechanisms involved in viral replication and pathogenesis. The endoplasmic reticulum (ER) stress response

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service