407241
Insulin Degrading Enzyme, His•Tag®, Rat, Recombinant, S. frugiperda
Synonym(s):
Insulysin, IDE
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About This Item
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Assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
specific activity
≥3 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. A metalloprotease that degrades insulin and a variety of other peptides including amyloid peptides.
Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.
Physical form
In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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