169756

Antithrombin III, Human Plasma

Single-chain glycoprotein present in normal serum at about 23 mg/100 ml.

• Synonym(s): Antithrombin III, Human Plasma, ATIII, Heparin cofactor, Factor Xa inhibitor
• CAS Number: 90170-80-2
• MDL number: MFCD00166632
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• form: solid
• specific activity: ≥5 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Single chain glycoprotein present in normal serum at about 23 mg/100 ml. Principal physiological inhibitor of thrombin. Present at higher levels in plasma than in serum because it complexes with thrombin during coagulation. Functions by inhibiting proteolytic enzymes involved in blood coagulation and fibrinolysis, including Factor Xa, plasmin, thrombin, and trypsin. Potency is strongly enhanced by heparin.

Single-chain glycoprotein present in normal serum at about 23 mg/100 ml. Principal physiological inhibitor of thrombin. Present at higher levels in plasma than in serum because it complexes with thrombin during coagulation. Functions by inhibiting proteolytic enzymes involved in blood coagulation and fibrinolysis, including Factor Xa, plasmin, thrombin, and trypsin. Potency is strongly enhanced by heparin.

Biochem/physiol Actions

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of antithrombin that will inhibit 1.0 unit of human plasma thrombin present in excess. Thrombin activity is measured using 500 µM tosyl-Gly-Pro-Arg-pNA as substrate in 100 mM Tris-HCl at 25°C, pH 8.2

Physical form

Lyophilized from 100 mM sodium citrate buffer, 20 mM Tris-HCl, pH 8.3.

Preparation Note

Please refer to label for lot-specific volume prior to lyophilization.

Analysis Note

single band by SDS-PAGE

Other Notes

Tsiang, M., et al. 1997. J. Biol. Chem. 272, 12024.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable