05-351
Anti-Fas Antibody, clone 7C10
clone 7C10, Upstate®, from rat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
7C10, monoclonal
species reactivity
mouse, bovine
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Fas(282488)
Specificity
Fas
Immunogen
recombinant protein containing the cytoplasmic domain of mouse Fas
Application
Anti-Fas Antibody, clone 7C10 is an antibody against Fas for use in IP & WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
routinely evaluated on RIPA lysates of mouse 3T3/A31 fibroblasts or membrane preparations of bovine brain
Target description
45kDa
Physical form
0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide
Format: Purified
Protein G Chromatography
Storage and Stability
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Fas-mediated apoptosis is suppressed by calf serum in cultured bovine luteal cells.
Dariusz J Skarzynski, Masami Shibaya, Yukari Tasaki, Anna Korzekwa, Shuko Murakami et al.
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Low-grade inflammation in adipose tissue and liver has been implicated in obesity-associated insulin resistance and type 2 diabetes. Yet, the contribution of inflammatory cells to the pathogenesis of skeletal muscle insulin resistance remains elusive. In a large cohort of obese
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Proceedings of the National Academy of Sciences of the United States of America, 114(14), 3648-3653 (2017-03-23)
Genetic lesions that activate KRAS account for ∼30% of the 1.6 million annual cases of lung cancer. Despite clinical need, KRAS is still undruggable using traditional small-molecule drugs/inhibitors. When oncogenic Kras is suppressed by RNA interference, tumors initially regress but
Regulation of luteal function and corpus luteum regression in cows: hormonal control, immune mechanisms and intercellular communication.
D J Skarzynski,G Ferreira-Dias,K Okuda
Reproduction in domestic animals = Zuchthygiene null
Hua Wang et al.
Molecular medicine reports, 13(3), 2892-2898 (2016-01-29)
Hyperoxia is a high risk factor for neurodevelopmental disorders and can cause nerve cell death. 17β‑Estradiol (E2) has been demonstrated as a neuroprotective agent. In the present study, the effect of hyperoxia on rat oligodendrocyte precursor cells (OPCs) in vivo
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