HPLC Analysis of Bovine Liver Extract on Mono Q^® 5/50 GL

column

Mono Q 5/50 GL, 5 cm x 5 mm I.D., 10 μm particles (5-4807)

mobile phase

[A] 50 mM diethanolamine, pH 8.8; [B] 50 mM diethanolamine + 1.0M sodium chloride, pH 8.8

flow rate

1.0 mL/min

detector

UV, 280 nm

injection

100 μL 1.6 mg protein