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Cloning of the 62-kilodalton component of basic transcription factor BTF2.

Science (New York, N.Y.) (1992-09-04)
L Fischer, M Gerard, C Chalut, Y Lutz, S Humbert, M Kanno, P Chambon, J M Egly
ABSTRAKT

Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.

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Sigma-Aldrich
TFIIH, p62 subunit human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)