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S7651

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Synonim(y):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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10 UNITS

397,00 zł

50 UNITS

1380,00 zł

397,00 zł

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Informacje o tej pozycji

Numer CAS:
9031-54-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-869-9
MDL number:
MFCD00132362
Numer WE:
3.1.4.12 (BRENDA, IUBMB)
Specific activity:
≥100 units/mg protein

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form

lyophilized powder

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Quality Level

200

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

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Ta pozycja
S9396567707L5135
Sphingomyelinase from Bacillus cereus lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

S7651

Sphingomyelinase from Bacillus cereus

Sphingomyelinase from Bacillus cereus buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Sigma-Aldrich

S9396

Sphingomyelinase from Bacillus cereus

Sphingomyelinase, Staphylococcus aureus

Sigma-Aldrich

567707

Sphingomyelinase, Staphylococcus aureus

L-Leucine Dehydrogenase from Bacillus cereus lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

Gene Information

-

Gene Information

-

specific activity

≥100 units/mg protein

specific activity

≥100 units/mg protein (Lowry)

specific activity

≥100 units/mg protein

specific activity

≥60 units/mg protein

form

lyophilized powder

form

buffered aqueous glycerol solution

form

liquid

form

lyophilized powder

mol wt

36873 Da

mol wt

34.2 kDa

mol wt

-

mol wt

245 kDa

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

General description

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme.[1] Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.[2]
Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages.[3] Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.[4]

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices[3]
  • for testing and confirming its silkworm-killing activity[4]
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures[5]

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease. [6]

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
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Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000419321
0000419323
0000419321
0000419323
0000287995

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity
Usui K, et al.
Microbial Pathogenesis, 46(2), 59-62 (2009)
Lipid bilayers containing sphingomyelins and ceramides of varying N-acyl lengths: a glimpse into sphingolipid complexity
Jimenez-Rojo N, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(1), 456-464 (2014)
Masataka Oda et al.
Biochimica et biophysica acta, 1798(6), 1073-1080 (2010-03-11)
Bacillus cereus sphingomyelinase (Bc-SMase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. We investigated the mechanism of this hemolysis in comparison to that induced by Clostridium perfringens alpha-toxin. Pertussis toxin, a Gi-specific inhibitor, N-oleoylethernolamine, a ceramidase inhibitor
Promotion of endocytosis efficiency through an ATP-independent mechanism at rat calyx of Held terminals
Yue HY, et al.
The Journal of Physiology, 595(15), 5265-5284 (2017)
Melissa Wasserstein et al.
Journal of inherited metabolic disease, 36(1), 123-127 (2012-06-22)
Niemann-Pick disease (NPD) due to acid sphingomyelinase deficiency is a lipid storage disease resulting from the accumulation of sphingomyelin, predominantly within cells of the monocyte-macrophage system. In contrast to other lysosomal storage disorders, skeletal involvement in NPD has not been
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Questions

  1. Hi for product number S7651, Sphingomyelinase from Bacillus cereus, once prepared how long can the Sphingomyelinase solution be kept and should it be kept at 2-8C or -20C?

    1 answer

    1. This product is stable for several days when stored refrigerated. Prepared solutions in aqueous buffers containing 50 % Glycerol are stable for extended periods when refrigerated.

      To review these product options please see the link below:
      https://www.sigmaaldrich.com/search/9031-54-3?focus=products&page=1&perpage=30&sort=relevance&term=9031-54-3&type=product

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