R0155

Anti-RanGAP1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-Ran-GTPase-Activating Protein 1
• Numer MDL: MFCD09265371
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~65 kDa
• reaktywność gatunkowa: human
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• stężenie: ~1 mg/mL
• metody: indirect immunofluorescence: 5-10 μg/mL using HeLa cells; indirect immunofluorescence: suitable; western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
• numer dostępu UniProt: P46060
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... RANGAP1(5905) ; mouse ... Rangap1(19387)

Opis

Opis ogólny

The unmodified form of RanGAP1 (65 kDa) is exclusively cytoplasmic, whereas the 90 kDa SUMO-1-modified form is associated with the cytoplasmic fibers of the nuclear pore complex (NPC).

Immunogen

synthetic peptide corresponding to amino acids 573-587 located at the C-terminus of human RanGAP1. The sequence is highly conserved (80% identity) in mouse RanGAP1.

Zastosowanie

Anti-RanGAP1 (C-terminal) antibody produced in rabbit has been used for immunoblotting and listeriolysin O (LLO) purification.

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Działania biochem./fizjol.

RanGAP1 (Ran GTPase Activating Protein 1, 65 kDa) is a key regulator of Ran activity, specifically inducing the GTPase activity of Ran. RanGAP1 is conjugated to the small ubiquitin-related modifier protein SUMO-1. The association of RanGAP1 with RanBP2 facilitates nuclear transport.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)