The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
PIC0004
Protease Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution
Synonym(s):
Protease inhibitor cocktail
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.28
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
Pricing and availability is not currently available.
Product Name
ReadyShield® Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution
description
Purification of Histidine-Tagged Proteins
Quality Segment
form
ready-to-use solution
quality
200
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
solubility
water: soluble
storage temp.
−20°C
General description
This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Tailored for use with Histidine fusion tagged proteins.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form DMSO solution |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging - | packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging pkg of 1 ml, 5 ml × |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| quality 200 | quality - | quality - | quality - |
| description Purification of Histidine-Tagged Proteins | description - | description - | description - |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the recommended storage temperature for ReadyShield® products?
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Do ReadyShield® inhibitor cocktails include EDTA?
1 answer-
No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
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