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Merck

P1585

PMA

for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

Synonim(y):

Phorbol 12-myristate 13-acetate, NF-κB Activator, PKC Activator, croton oil, phorbol ester, protein kinase C activator, 12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, TPA

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Wybierz wielkość

1 MG

751,00 zł

5 MG

1210,00 zł

10 MG

4530,00 zł

25 MG

5310,00 zł

751,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C36H56O8
Numer CAS:
16561-29-8
Masa cząsteczkowa:
616.83
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24898219
MDL number:
MFCD00036736
Beilstein/REAXYS Number:
2407201
Assay:
≥99% (HPLC)
Form:
(Lyophilisate)
Quality level:
200
Storage condition:
protect from light

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grade

Molecular Biology

Quality Level

200

assay

≥99% (HPLC)

form

(Lyophilisate)

mol wt

616.83  g/mol

storage condition

protect from light

solubility

DMSO: soluble (DMSO solutions can be stored dark at −20 °C for at least six months.), ethanol: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions)

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)C(C=C(CO)C[C@@]3(O)C2C=C(C)C3=O)C4C(C)(C)[C@]14OC(C)=O

InChI

1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

InChI key

PHEDXBVPIONUQT-RGYGYFBISA-N

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Pokaż różnice

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Ta pozycja
P8139524400524390
PMA for use in molecular biology applications, ≥99% (HPLC), Molecular Biology

Sigma-Aldrich

P1585

PMA

Phorbol 12-myristate 13-acetate ≥99% (TLC), film or powder

Sigma-Aldrich

P8139

Phorbol 12-myristate 13-acetate

Phorbol-12-myristate-13-acetate Phorbol-12-myristate-13-acetate, CAS 16561-29-8, is the most common phorbol ester. Activates PKC at nanomolar concentrations.

Sigma-Aldrich

524400

Phorbol-12-myristate-13-acetate

Phorbol-12,13-dibutyrate Strong irritant for mouse skin, but only moderately active as a tumor promoter.

Sigma-Aldrich

524390

Phorbol-12,13-dibutyrate

assay

≥99% (HPLC)

assay

≥99% (TLC)

assay

≥98% (HPLC)

assay

≥99% (HPLC)

form

(Lyophilisate)

form

film or powder

form

solid

form

solid (crystalline)

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

616.83  g/mol

mol wt

-

mol wt

-

mol wt

-

storage condition

protect from light

storage condition

-

storage condition

OK to freeze

storage condition

OK to freeze

General description

Phorbol 12-myristate 13-acetate (PMA) can induce the activity of PLD1 (phospholipase D1) but not PLD2 (phospholipase D2).

Through the protein kinase C (PKC) signaling pathway, PMA is a tumor promoter associated with:

  • gene transcription
  • cell growth
  • cell differentiation
  • programmed cell death
  • immune pathway
  • receptor desensitization

Phorbol 12-myristate 13-acetate is used to study:

  • growth factors
  • hormones
  • cytokines-mediated regulation of cellular growth and differentiation
PMA (Phorbol 12-myristate 13-acetate) is an activator of protein kinase C (PKC) and a known tumor promoting agent. PMA also exhibits clastogenic (chromosome-breaking) activity.[1]

Application

Suitable for
  • molecular biology studies as standard
  • analytical studies of tumor promoters
  • promotion of cell growth
PMA has been used:
  • for the detection of intracellular cytokines
  • in CellROX oxidative stress assay
  • for Huh7 cell stimulation to investigate dipeptidyl peptidase 9 (DPP9) re-distribution
  • as a positive control in B-cell receptor (BCR) stimulation of chronic lymphocytic leukaemia (CLL) cells
  • in the differentiation of monocytes (THP -1) into macrophages

Biochem/physiol Actions

Potent tumor promoter; activates protein kinase C (PKC) in vivo and in vitro.

Features and Benefits

  • Stimulation of interleukin 2 production of Jurkat cells
  • T-cell stimulation
  • Protein kinase C (PKC) activator
  • NF-κB Activator
  • Promotion of cell growth
  • Used in analytical studies of tumor promoters
  • Molecular Biology grade

Analysis Note

  • Stimulation of interleukin 2 production of Jurkat cells
  • T-cell stimulation
  • Protein kinase C (PKC) activator
  • NF-κB Activator
  • Promotion of cell growth
  • Used in analytical studies of tumor promoters
  • Molecular Biology grade

Disclaimer

Photosensitive, protect from light.
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Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Klasa składowania

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
0000541374
0000524833
0000524833
0000529127
0000529127

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

The immune responses of central and effector memory BCG-specific CD4+ T cells in BCG-vaccinated PPD+ donors were modulated by Treg cells
Li L, et al.
Immunobiology, 216(4), 477-484 (2011)
Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action.
I Emerit et al.
Nature, 293(5828), 144-146 (1981-09-10)
Biological properties and measurement of phospholipase D activation by ADP-ribosylation factor (ARF)
Cockcroft S, et al.
Methods in Enzymology, 329, 355-372 (2001)
Identification of a potential biomarker for FABP4 inhibition: the power of lipidomics in preclinical drug testing
Suhre K, et al.
Journal of Biomolecular Screening, 16(5), 467-475 (2011)
Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen
Kashuba E, et al.
Journal of Proteomics, 91(2), 478-485 (2013)
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Powiązane treści

Protokół odczynnika do biologii molekularnej 12-myristanu 13-octanu phorbolu

Aktywacja limfocytów T jest zwykle wywoływana przez interakcję receptora powierzchniowego komórki z jego specyficzną cząsteczką ligandu

Phorbol 12-myristate 13-acetate Molecular Biology Reagent Protocol

T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule

Data Sheet - P1585

PMA, through its activation of PKC, can activate T-cells and stimulate low-level production of IL-2. When Jurkat cells are stimulated by PMA and a co-stimulator, such as PHA, IL-2 production is strongly enhanced2.

Numer pozycji handlu globalnego

SKUNUMER GTIN
P1585-10MG04061835574568
P1585-1MG04061835574575
P1585-25MG04061835574582
P1585-5MG04061842154661

Questions

1–10 of 11 Questions  

  1. I received the product of PMA cat# P1585 in solution. But what is the product of PMA cat#1585 concentration? or volume? without concentration or volume can't be calculate the further dilution.

    1 answer

    1. The stock concentration is 100ug/mL.

      Helpful?

  2. How can I resuspend properly the PMA into the shipped vial? Can I open the vial?

    1 answer

    1. The packaging consists of an amber serum vial sealed with a septum and an aluminum crimp cap. The product is typically dissolved in the vial by injecting the appropriate volume of the selected solvent through the septum using a syringe. Additional solubility and solution stability information can be reviewed in the datasheet available for the general use product, P8139. See the link below to review these recommendations:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/816/596/p8139pis.pdf

      Helpful?

  3. What is the stock concentration of the PMA when adding DMSO. and How much of DMSO do we add?

    1 answer

    1. Suitability Assay - 2.5 ml of Jurkat cells (1 x 10e6 cells/mL) and 2.5 mLof fresh media (RPMI 1640 + 10% fetal calf serum containing 10 ml/l penicillin-streptomycin) were added to 25 cm2 culture bottles.
      The following additions were made in duplicate.
      a. Control - no additions
      b. 1 µg/ml PHA Control - add 10 µl PHA stock solution (0.5 mg/ml PHA in filter-sterilized PBS)
      c. 1 µg/ml PHA + 50 ng/ml PMA – 10 µl PHA stock solution + 2.5 µl PMA stock solution (100 µg/ml PMA in DMSO).

      As stated in part c. - the PMA stock solution is 100µg/ml PMA in DMSO.

      Please see the datasheet for more details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/311/794/p1585dat.pdf

      Helpful?

  4. What is the stability of stock solutions of Phorbol 12-myristate 13-acetate, Product P1585, and how should I store them?

    1 answer

    1. Stock solutions in DMSO can be stored in the dark as aliquots at -20°C for at least six months.

      Helpful?

  5. How is Product P1585, Phorbol-12-myristate-13-acetate, packaged?

    1 answer

    1. P1585-1MG, P1585-10MG and P1585-25MG come to us pre-packaged by our supplier; all three sizes are packaged the same way. The packaging consists of an amber serum vial with a septum and an aluminum crimp cap. The septum has a PTFE (Teflon) liner, so the material inside the vial could only come in contact with glass and Teflon.

      Helpful?

  6. There isn't any powder in the Phorbol 12-myristate 13-acetate, Product P1585, vial?

    1 answer

    1. The product is usually a clear film on the walls of the vial and may not be easily visible.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the storage temperature for Phorbol 12-myristate 13-acetate, Product P1585?

    1 answer

    1. Phorbol 12-myristate 13-acetate should be stored at -20°C.

      Helpful?

  9. How much Phorbol 12-myristate 13-acetate, Product P1585, product should I use?

    1 answer

    1. A concentration of 50 ng/ml was used on Jurkat cells.

      Helpful?

  10. How can I dissolve Phorbol 12-myristate 13-acetate, Product P1585?

    1 answer

    1. Phorbol 12-myristate 13-acetate (PMA) is soluble in DMSO at a 20 mM concentration.  It is also soluble in ethanol.

      Helpful?

1–10 of 11 Questions  

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