Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

Synonim(y):

Rapid mammalian nuclei isolation kit

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Kod UNSPSC:
12352207
NACRES:
NA.32

zastosowanie

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

okres trwałości

1 yr at 2‑8 °C

opakowanie

pkg of 1 kit

Zastosowanie

cell analysis

obecność zanieczyszczeń

nuclease and protease, free

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Opis ogólny

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Zastosowanie

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Działania biochem./fizjol.

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Inne uwagi

All procedures should be carried out on ice or 2-8°C.
This page may contain text that has been machine translated.

produkt powiązany

Numer produktu
Opis
Cennik

08168

Timestrip Plus™ 8 °C

Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Alan Selewa et al.
Scientific reports, 10(1), 1535-1535 (2020-02-01)
A comprehensive reference map of all cell types in the human body is necessary for improving our understanding of fundamental biological processes and in diagnosing and treating disease. High-throughput single-cell RNA sequencing techniques have emerged as powerful tools to identify
Baochen Shi et al.
BMC genomics, 10, 92-92 (2009-02-27)
A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory
Wyświetl wszystkie powiązane dokumenty

Produkty

Izolacja organelli

Wirowanie oddziela organelle na podstawie rozmiaru, kształtu i gęstości, ułatwiając frakcjonowanie subkomórkowe w różnych próbkach.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej