One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.
N7271
α(2→3) Neuraminidase from Streptococcus pneumoniae
buffered aqueous solution
Synonim(y):
Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase
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0.2 UNIT
4910,00 zł
4910,00 zł
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Biological source:
Streptococcus pneumoniae
Concentration:
≥5 units/mL
Przejdź do
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Pozwól nam pomócbiological source
Streptococcus pneumoniae
form
buffered aqueous solution
concentration
≥5 units/mL
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
Streptococcus pneumoniae R6 ... nanB(933504)
Powiązane kategorie
1 of 4
Ta pozycja | N8271 | N6514 | N7885 |
|---|---|---|---|
| biological source Streptococcus pneumoniae | biological source - | biological source Vibrio cholerae | biological source - |
| Gene Information Streptococcus pneumoniae R6 ... nanB(933504) | Gene Information - | Gene Information - | Gene Information - |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| concentration ≥5 units/mL | concentration - | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Biochem/physiol Actions
Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 50 mM sodium phosphate, pH 7.5
Preparation Note
Expressed in glycosidase-free hosts.
Other Notes
One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Nenad Macesic et al.
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Datasheet
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What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
Helpful?
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What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?
1 answer-
The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.
Helpful?
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What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?
1 answer-
The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.
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