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MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonim(y):

Citrate Synthase Test Kit

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About This Item

Numer WE:
Kod UNSPSC:
12161503
NACRES:
NA.84
Informacje o cenach i dostępności nie są obecnie dostępne.

metoda wykrywania

colorimetric

powiązane choroby

endocrinological disorders, diabetes; cancer

temp. przechowywania

−20°C

informacje o genach

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Opis ogólny

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.[1]

Zastosowanie

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.[2]

Przydatność

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Zasada

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.
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Tylko elementy zestawu

Numer produktu
Opis

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3


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Background: Identification of the mechanisms underlying mitochondrial dysfunction is key to understanding the pathophysiology of acute injuries such as cardiac arrest (CA); however, effective methods for measurement of mitochondrial function associated with mitochondrial isolation have been debated for a long
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
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Questions

  1. Is the sensitivity information for the CS0720 (Citrate Synthase Assay Kit) available?

    1 answer
    1. The lower concentration tested for the product CS0720 (Citrate Synthase Assay Kit) is 1u/µgP

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 0
    1 out of 5 stars.

    This actually measures total deacetylation activity, not specific to CS

    The mechanism for the assay kit is the deacetylation of Acetyl-CoA to CoA. There are multiple enzymes can do this, not just CS. This assay kit doesn't have a good control for the background non-CS-related deacetylation step, so it is not the right product to measure CS activity. CS0720 is the CS specific assay kit to buy.

    Helpful?

    1. Response from MilliporeSigma:

      MAK193 has been discontinued, CS0720 is its recommended replacement. CS0720 includes a positive control (which MAK193 lacked). CS0720 provides the Acetyl Coenzyme A and the Oxaloacetic acid needed for Citrate Synthase to produce Citrate.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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