E6781

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-4E-T, Anti-Clast4, Anti-Eukaryotic translation initiation factor 4E nuclear import factor 1, Anti-Eukaryotic translation initiation factor 4E transporter, Anti-eIF4E-T
• Numer MDL: MFCD09753854
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen 100 kDa
• reaktywność gatunkowa: human
• metody: immunoprecipitation (IP): 5-10 μL using HeLa cell lysates; indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde-fixed HEK-293T cells; western blot: 1-2 μg/mL using HeLa cell lysates
• numer dostępu UniProt: Q9NRA8
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... EIF4ENIF1(56478)

Opis

Opis ogólny

Eukaryotic translation initiation factor 4E nuclear import factor 1 (eIF4ENIF1) comprises two leucine-rich nuclear export signals and a bipartite nuclear localization signal. eIF4ENIF1 gene is mapped to human chromosome 22q12.2 and is expressed in the human ovary.

Specyficzność

Anti-eIF4ENIF1(N-terminal) specifically recognizes human eIF4ENIF1.

Immunogen

synthetic peptide corresponding amino acids 1-19 of human eIF4ENIF1, conjugated to KLH via a C-terminal added cysteine residue. This sequence is specific to human.

Zastosowanie

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit is suitable for the following applications:

• Immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates
• Indirect immunofluorescence at a concentration of 5-10μg/mL using paraformaldehyde-fixed HEK-293T cells
• Western blotting at a concentration of 1-2μg/mL using HeLa cell lysates

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Działania biochem./fizjol.

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that bind the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. Its interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies.

eIF4ENIF1 (also known as 4E-T or 4E-transporter) colocalizes with other mRNA binding/processing proteins to processing (P)-bodies. Its interaction with eIF4E is essential for targeting of mRNAs to P-bodies. eIF4ENIF1 competes with eIF4G for binding to eIF4E and preventing the formation of the eIF4F complex thus inhibits translation. Together with 4E-BPs, it participates in cell response to prolonged hypoxia, sequestering eIF4E, and affecting gene expression. A nonsense mutation in the eIF4ENIF1 gene is implicated in the Primary Ovarian Insufficiency.

Postać fizyczna

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)