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D5905

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

Synonym(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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Size/SKUAvailabilityPrice
50 tabs

Estimated to ship TODAYfromMILWAUKEE

$458.00
100 tabs

Estimated to ship TODAYfromMILWAUKEE

$783.00
5 x 100 tabs

Estimated to ship TODAYfromMILWAUKEE

$2,960.00

About This Item

Linear Formula:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Molecular Weight:
360.11
NACRES:
NA.83
PubChem Substance ID:
24893981
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:
MFCD00012969
Beilstein/REAXYS Number:
1212988

$458.00

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Product Name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Quality Segment

200

form

tablet

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

storage temp.

−20°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

Application

Reagent for spectrophotometric determination of selenium.
3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.
3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

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3,3′-Diaminobenzidine tetrahydrochloride tablet, 10 mg substrate per tablet

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D5905

3,3′-Diaminobenzidine tetrahydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride tablet, 1 mg substrate per tablet

Sigma-Aldrich

T3405

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

3,3′-Diaminobenzidine 97% (HPLC)

Sigma-Aldrich

D8001

3,3′-Diaminobenzidine

3,3′-Diaminobenzidine 97%

Sigma-Aldrich

D12384

3,3′-Diaminobenzidine

solubility

water: 1 tablet/15 mL (may contain small insoluble particles)

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

ppe

Eyeshields, Gloves, type N95 (US)

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