If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
CS0003
Zestaw do oznaczania aktywności acetylocholinoesterazy
Synonim(y):
Zestaw testowy AChE
Wybierz wielkość
2120,00 zł
2120,00 zł
Informacje o tej pozycji
Przejdź do
usage
sufficient for 200 colorimetric test
shipped in
dry ice
storage temp.
−20°C
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Ta pozycja | |||
|---|---|---|---|
| usage sufficient for 200 colorimetric test | usage sufficient for 100 colorimetric tests | usage sufficient for 100 colorimetric tests | usage sufficient for 100 colorimetric or fluorometric tests |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in - | shipped in dry ice |
General description
Zakres wykrywania: 10-600 jednostek/litr aktywności acetylocholinoesterazy.
Czas wykrywania: 5-10 minut
Acetylocholinoesteraza (AChE) jest hydrolazą serynową występującą w połączeniach nerwowo-mięśniowych i cholinergicznych synapsach mózgu. AChE hydrolizuje estry choliny. Aktywność acetylocholinoesterazy jest związana z funkcją odpowiedzi nerwowej. Jej główną rolą biologiczną jest zakończenie transmisji impulsów w synapsach cholinergicznych poprzez szybką hydrolizę neuroprzekaźnika acetylocholiny (ACh) do octanu i choliny.
Trucizny fosforoorganiczne (OP) tworzą wiązanie kowalencyjne z resztą serynową w miejscu aktywnym AChE, a zatem są silnymi nieodwracalnymi inhibitorami AChE.
Inhibitory AChE są stosowane w leczeniu różnych zaburzeń nerwowo-mięśniowych i stanowią pierwszą generację leków stosowanych w leczeniu choroby Alzheimera.
Inhibitory AChE hamują rozkład ACh przez enzym cholinesterazę, zwiększając zarówno poziom, jak i czas działania neuroprzekaźnika. W zależności od sposobu działania, inhibitory AChE można podzielić na dwie grupy: nieodwracalne i odwracalne. Odwracalne inhibitory, konkurencyjne lub niekonkurencyjne, mają głównie zastosowania terapeutyczne, podczas gdy efekty toksyczne są związane z nieodwracalnymi modulatorami aktywności AChE.
Application
Umożliwia badanie przesiewowe pod kątem inhibitorów acetylocholinoesterazy
Features and Benefits
- Prosta, szybka, czuła i bezpośrednia procedura pomiaru poziomu aktywności acetylocholinoesterazy w różnych próbkach, takich jak surowica i osocze, tkanki lub lizaty komórkowe, umożliwia badanie przesiewowe w kierunku inhibitorów acetylocholinoesterazy.
- Kalkulator pomocniczy (Kliknij tutaj, aby pobrać plik kalkulatora excel): Obliczy potrzebne odczynniki zgodnie z potrzebami eksperymentalnymi i przeanalizuje wyniki na podstawie danych eksperymentalnych!
Other Notes
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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Powiązane treści
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can U or V bottom plates be used for taking the reading?
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This kit requires the use of 96-well, clear, flat-bottom plates. Please see the link below to review the product Technical Bulletin for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/312/814/cs0003bul-ms.pdfHelpful?
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1. Is this kit suitable for estimation of red blood cell acetylcholinesterase activity? 2. Can Triton be used for haemolysate preparation?
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This product is suitable for assaying acetylcholinesterase activity in cell lysates such as red blood cell hemolysates. Triton X-100 has not been tested for use in this kit. While there are published protocols that do suggest up to 1% Triton X-100, the impact if this detergent on results has not been evaluated. For cell lysates, it is recommended to prepare samples by brief sonication or by homogenization in 0.1 M phosphate buffer (pH 8.0), followed by centrifugation at 14,000 rpm for 5 minutes. The clear supernatant is used for the assay. The Assay Buffer included with the kit should be used for sample dilution.
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Is Acetylcholinesterase compatible with the Roche platform, specifically the COBAS 6000?
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The product in question, an acetylcholinesterase enzyme or kit, is not compatible with the ROCHE Cobas 6000, as it is a clinical analyzer for clinical chemistry or immunochemistry. All acetylcholinesterase products available are intended for research purposes only and not for human or clinical use.
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Is this kit only for acetylcholinesterase or also tests for butylcholinesterase? Is this testing total cholinesterase activity or just AChE activity?
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Product CS0003 only measures Acetylcholinesterase not butylcholinesterase and measures AChE activity.
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What is the difference between the two Acetylcholinesterase Activity Assay Kits MAK119 and CS0003?
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Product CS0003 and MAK119 are produced by different manufacturers. In addition, CS0003 is sufficient for 200 tests while product MAK119 is sufficient for 100 tests. The principles and detection range are the same for both products.
The following is true for product CS0003:
- Direct procedure for measuring AChE levels in a variety of samples such as serum and plasma.
- Based on the Ellman method in which thiocholine, produced by AChE, reacts with 5,5'-dithiobis (2-nitrobenzoic acid) to form a colorimetric (412 nm) product, proportional to the AChE activity present.
- The AChE activity is measured without the use of a calibration curve. The AChE Positive Control in the kit can be used to screen for AChE inhibitors.
- This kit has a linear range of 10-600 units/liter of AChE activity.
The following is true for product MAK119:
- Direct assays of acetylcholinesterase activity in blood, serum, plasma, and other biological samples.
- Based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
- Detection range 10 to 600 U/L AChE activity in 96-well plate assay.Helpful?
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Is product CS0003, Acetylcholinesterase Activity Assay Kit, suitable for use with whole blood? What is the recommended sample preparation protocol, e.g. the proper amount to dilute whole blood for assay?
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Yes, kit CS0003 is suitable to test ACHE activity in whole blood. It is recommended to begin with (at least) 1:100 working dilution and progress from there.
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