GH1

89072610, rat brain, Epithelial

• Kod UNSPSC: 41106514

Właściwości

• product name: GH1, 89072610
• pochodzenie biologiczne: rat brain
• tryb wzrostu: Semi-adherent
• kariotyp: Hypodiploid, modal no. 40.
• morfologia: Epithelial
• produkty: Rat growth hormone, lactogenic hormones.
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Rat Wistar-Furth pituitary tumour

Opis linii komórkowej

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Zastosowanie

Hormone studies

pożywka hodowlana

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Inne uwagi

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