Jurkat E6.1 Cell Line human

88042803, human blood (leukemic T-cell lymphoblast), Not specified

• Synonim(y): Jurkat Cells, Jurkat Clone E6-1 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: Jurkat E6.1 Cell Line human, 88042803, from human blood(leukemic T-cell lymphoblast)
• pochodzenie biologiczne: human blood (leukemic T-cell lymphoblast)
• tryb wzrostu: Suspension
• kariotyp: Pseudodiploid, modal No 46
• morfologia: Not specified
• produkty: Interleukin 2 (IL-2), human alpha interferon
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human leukaemic T cell lymphoblast

Opis linii komórkowej

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Zastosowanie

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:

• study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
• determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
• study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Inne uwagi

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