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C-26130

Renal Epithelial Cell Growth Medium 2

Kit including Basal Medium and SupplementPack, 500 ml

Synonym(s):

Epithelial Growth Medium, Medium for Renal Cells, Renal Cell Medium

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
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form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Renal Epithelial Cell Growth Medium 2 Kit

Application

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

Packaging

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Preparation Note

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Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 36 ng / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 4 pg / mlTransferrin (recombinant human): 5 µg / ml
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Analysis Note

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

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This Item
C-26030C-26235C-39606
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

aqueous solution

form

aqueous solution

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

packaging

pkg of 500 ml

packaging

pkg of 500 ml

packaging

pkg of 500 ml

packaging

pkg of 1 vial

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


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Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Storage Class

3 - Flammable liquids



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