C1345

Catalase from bovine liver

powder, suitable for cell culture, 2,000-5,000 units/mg protein

• Synonim(y): H₂O₂:H₂O₂ oxidoreductase
• Numer CAS: 9001-05-2
• Numer EC enzymu: 1.11.1.6 (BRENDA, IUBMB)
• Numer WE: 232-577-1
• Numer MDL: MFCD00081483
• Kod UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.75

Właściwości

• pochodzenie biologiczne: bovine liver
• Poziom jakości: 200
• linia produktu: BioReagent
• Formularz: powder
• aktywność właściwa: 2,000-5,000 units/mg protein
• masa cząsteczkowa: tetramer ~250 kDa
• metody: cell culture | mammalian: suitable
• punkt izoelektryczny: 5.4
• numer dostępu UniProt: P00432
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• ciąg SMILES: O(CC)C(=O)c1ccc(cc1)O
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• Klucz InChI: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• informacje o genach: cow ... CAT(280743)

Opis

Opis ogólny

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.[1]

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Zastosowanie

Catalase from bovine liver is used for the following applications:

• Gel filtration[2]
• Analytical filtration on Ultrogel and glycerol gradient centrifugation[3]
• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor[4]
• Enzyme assays[5][6]
• Alcohol oxidase activity[7]

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Działania biochem./fizjol.

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Przestroga

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Definicja jednostki

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Uwaga dotycząca przygotowania

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)