C112000

DMT-dC(tac) Phosphoramidite

• Synonim(y): DMT-dC(tac) amidite, N-[2-[4-(1,1-dimethylethyl)phenoxy]acetyl]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxycytidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
• Wzór empiryczny (zapis Hilla): C₅₁H₆₂N₅O₉P
• Numer CAS: 149989-59-3
• Masa cząsteczkowa: 920.04
• NACRES: NA.51
• UNSPSC Code: 12352202
• Assay: ≥98% (³¹P-NMR), ≥98.0% (reversed phase HPLC)
• Form: powder

Właściwości

• InChI: 1S/C49H58N5O7P/c1-35(2)54(36(3)4)62(59-31-17-29-50)61-42-32-46(53-30-28-44(52-47(53)56)51-45(55)34-57-41-26-24-37(25-27-41)48(5,6)7)60-43(42)33-58-49(38-18-11-8-12-19-38,39-20-13-9-14-21-39)40-22-15-10-16-23-40/h8-16,18-28,30,35-36,42-43,46H,17,31-34H2,1-7H3,(H,51,52,55,56)/t42-,43+,46+,62?/m0/s1
• InChI key: NHKNJNORWFDRBB-ROEGVEHFSA-N
• SMILES string: CC(C)N(C(C)C)P(OCCC#N)O[C@H]1C[C@@H](O[C@@H]1COC(c2ccccc2)(c3ccccc3)c4ccccc4)N5C=CC(NC(=O)COc6ccc(cc6)C(C)(C)C)=NC5=O
• type: for DNA synthesis
• product line: Proligo Reagents
• assay: ≥98% (³¹P-NMR), ≥98.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• λ: conforms (UV/VIS Identity)
• nucleoside profile: base: deoxycytidine; base protecting group: TAC; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast
• storage temp.: -10 to -25°C
• Quality Level: 200

Opis

General description

DMT-dC(tac) Phosphoramidite is a TAC Protected Phosphoramidite. Substitution of standard protecting groups with the labile TAC (tert.-butylphenoxyacetyl) protecting group results in ultra-fast and easy deprotection under mild conditions, suitable for oligonucleotides with base labile monomers and reporters as well as in-situ synthesis schemes on glass surfaces.

Other Notes

• The deprotection of oligonucleotide synthesis products with the AMAreagent is ultra-fast: complete deprotection requires 10 minutes at 65 °C
• Side reactions at C-monomers through transamination are eliminated
• Not compatible with some base-labile modified nucleosides
• dC(tac)-phosphoramidite can directly substitute for dC(bz)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis.Acetonitrile is used to dissolve phosphoramidite. The standard aceticanhydride capping reagent can be employed.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable