B7632

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

Name: N-Benzoyl-Phe-Val-Arg-<I>p</I>-nitroanilide hydrochloride
Brand: SIGMA
Price: 1050 PLN

chromogenic, protease substrate, ≥98% (TLC), powder

Synonim(y):

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride

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5 MG

1050,00 zł

10 MG

1810,00 zł

50 MG

6320,00 zł

1050,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):

C₃₃H₄₀N₈O₆ · HCl

Numer CAS:

54799-93-8

Masa cząsteczkowa:

681.18

NACRES:

NA.32

PubChem Substance ID:

24892011

UNSPSC Code:

12352204

MDL number:

MFCD00038397

Beilstein/REAXYS Number:

3027332

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Nazwa produktu

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Quality Level

200

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

SMILES string

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

assay

≥98% (TLC)

form

powder

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

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Ta pozycja	B3279	B2133	B4875
Sigma-Aldrich B7632 N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride	Sigma-Aldrich B3279 N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride	Sigma-Aldrich B2133 N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride	Sigma-Aldrich B4875 Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride
assay ≥98% (TLC)	assay >98% (TLC)	assay ≥97% (HPLC)	assay ≥98%
solubility methanol: 20 mg/mL, clear, colorless to light yellow	solubility acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow	solubility methanol: 25 mg/mL	solubility DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

A substrate for trypsin, thrombin and reptilase.

N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.[1]

Application

-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae[2]
for the thrombin, recombinant and native batroxobin from snake venom[3]
for fibrinolytic enzyme aprE2 in amidolytic activity assay[4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Chromogenic methods in coagulation diagnostics.

S Rosén

Hamostaseologie, 25(3), 259-266 (2005-08-23)

Chromogenic peptide substrates were developed more than 30 years ago. Although the use of chromogenic substrate methods in coagulation and fibrinolysis diagnostics was not as rapidly implemented as initially believed, they are now well established for several analytes such as

[Clinical evaluation of prothrombin assay using chromogenic peptide substrates (author's transl)].

Y Akiyama et al.

Rinsho byori. The Japanese journal of clinical pathology, 28(11), 1131-1135 (1980-11-01)

Molecular cloning and characterization of serine protease inhibitor from food-borne nematode, Gnathostoma spinigerum.

Anusorn Tinyou et al.

Acta tropica, 204, 105288-105288 (2019-12-08)

Gnathostoma spinigerum is a causative agent of human gnathostomiasis and infects people residing in endemic areas as well as travelers. Cutaneous and visceral larval migrants cause clinical manifestations, resulting in severe morbidity and mortality. To survive in hosts, these parasites

Hydrolytic enzymes of leaf-cutting ant fungi.

Milton Erthal et al.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 152(1), 54-59 (2008-10-14)

The production of enzymes and the colonization of leaves by Leucoagaricus gongylophorus were investigated to further understand the digestive interactions of leaf-cutting ant colonies. The enzymes detected were indicative of a saprophytic origin of this fungus, producing all the enzymes

Overexpression of aprE2, a fibrinolytic enzyme gene from Bacillus subtilis CH3-5, in Escherichia coli and the properties of AprE2.

Seon-Ju Jeong et al.

Journal of microbiology and biotechnology, 24(7), 969-978 (2014-04-20)

The aprE2 gene with its prosequence from Bacillus subtilis CH3-5 was overexpressed in Escherichia coli BL21(DE3) by using plasmid pET26b(+). After IPTG induction, active and mature AprE2 was produced when cells were grown at 20°C, whereas inactive and insoluble enzyme

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Protokoły

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride