B6024

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

tablet, suitable for identification of lac+ bacterial colonies

• Synonim(y): 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Wzór empiryczny (zapis Hilla): C₁₄H₁₅BrClNO₆
• Numer CAS: 7240-90-6
• Masa cząsteczkowa: 408.63
• Beilstein: 1402009
• Numer WE: 230-640-8
• Numer MDL: MFCD00005666
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 57653950
• NACRES: NA.83

Właściwości

• product name: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet
• Postać: tablet
• przydatność: suitable for identification of lac⁺ bacterial colonies
• temp. przechowywania: −20°C
• ciąg SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• Klucz InChI: OPIFSICVWOWJMJ-AEOCFKNESA-N

Opis

Opis ogólny

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Zastosowanie

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Działania biochem./fizjol.

X-gal is routinely used to detect transgene activity in situ.

Ilość

Contains 5 mg substrate per tablet

Rekonstytucja

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)