41969

5-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

• Empirical Formula (Hill Notation): C₃₇H₃₃N₃O₇
• CAS Number: 209734-74-7
• Molecular Weight: 631.67
• NACRES: NA.32
• PubChem Substance ID: 329756168
• UNSPSC Code: 12352116
• MDL number: MFCD07437880

Properties

• product line: BioReagent
• Quality Segment: 100
• form: powder
• solubility: DMF: soluble, acetonitrile: soluble
• fluorescence: λ_ex 575 nm; λ_em 600 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: [O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O
• InChI: 1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2
• InChI key: BTTBJYLMDASSAS-UHFFFAOYSA-N

Description

Application

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.[1][2]

Other Notes

Labeling reagent for prepn. of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing[1][3][2]

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves