18507

Atto 565 maleimide

BioReagent, suitable for fluorescence

• Empirical Formula (Hill Notation): C₃₇H₃₇ClN₄O₁₀
• Molecular Weight: 733.16
• UNSPSC Code: 12352111
• NACRES: NA.32
• MDL number: MFCD04040675

Properties

• product line: BioReagent
• Quality Segment: 200
• assay: >90.0% (HPLC)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm, 565 nm
• fluorescence: λ_ex 563 nm; λ_em 592 nm in 0.1 M phosphate pH 7.0
• λ: (ethanol with 0.1% trifluoroacetic acid)
• UV absorption: λ: 560-566 nm A_max
• suitability: corresponds for coupling to thiols, suitable for fluorescence
• storage temp.: −20°C

Description

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)