14562

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester

BioReagent, suitable for fluorescence

• Synonym(s): BCECF-AM
• Empirical Formula (Hill Notation): C₃₉H₃₆O₁₉
• Molecular Weight: 808.69
• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD01862636

Properties

• product line: BioReagent
• Quality Segment: 100
• form: solid
• solubility: DMF: soluble, DMSO: soluble, acetonitrile: soluble
• fluorescence: λ_ex 482 nm; λ_em 528 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C

Description

Application

An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues[1][2][3], intercellular spaces[4][5], mammalian cells[6][7][8], plant cells[9], bacteria[10][11], and yeast[12][13]. Also, employed to investigate in assays for cellular functional properties such as adhesion[14][15][16][17][18][19], multi-drug resistance[20][21][22], viability and cytotoxicity[23][24][25][26][27], apoptosis[28] and chemotaxis[15].

Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)