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E7889

Ethylenediaminetetraacetic acid disodium salt solution

Molecular Biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Synonim(y):

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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Gabaryty przesyłkiSKUDostępnośćCena netto
100 mL
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619,00 zł
1 L
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1670,00 zł

Informacje o tej pozycji

Wzór liniowy:
[-CH2N(CH2CO2Na)CH2CO2H]2
Numer CAS:
Masa cząsteczkowa:
336.21
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3822669

619,00 zł


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grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

suitability

suitable for molecular biology

application(s)

microbiology

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

For additional information on our range of Biochemicals, please complete this form.
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Ta pozycja
03690ED4SSE6635
grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

Sigma Grade

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

application(s)

microbiology

application(s)

-

application(s)

-

application(s)

-

pH

7.5-8.5

pH

8.0±0.2

pH

-

pH

-

form

liquid

form

liquid

form

crystalline

form

powder

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-


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pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protokoły

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Produkty

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.


Marta Filipiak et al.
Fish physiology and biochemistry, 38(2), 355-362 (2011-05-27)
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken
Yi-Yuan Ke et al.
Foods (Basel, Switzerland), 10(1) (2021-01-21)
Isothiocyanates (ITCs) are important functional components of cruciferous vegetables. The principal isothiocyanate molecule in broccoli is sulforaphane (SFN), followed by erucin (ERN). They are sensitive to changes in temperature, especially high temperature environments where they are prone to degradation. The
Gary Reynolds et al.
Science (New York, N.Y.), 371(6527) (2021-01-23)
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic



Numer pozycji handlu globalnego

SKUNUMER GTIN
E7889-1L04061838110152
E7889-100ML04061838255334

Questions

1–8 of 8 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can a solution of EDTA disodium salt, Product E7889, be autoclaved?

    1 answer
    1. Yes, solutions of EDTA may be autoclaved.

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  4. How many divalent cations does Product E7889, EDTA disodium salt, complex with?

    1 answer
    1. EDTA forms 1:1 complex with divalent cations.

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  5. What is the pH of E7889, EDTA disodium salt solution?

    1 answer
    1. E7889 has a pH specification of 7.5 to 8.5 at 25 °C.

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  6. At what concentration should I use Product E7889, EDTA disodium salt, as an anticoagulant?

    1 answer
    1. The optimal concentration of EDTA to be used as an anticoagulant is 1.5 mg of EDTA disodium salt per ml of blood or 1-2 drops of a 2% EDTA solution per ml of blood.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What type of biological activity does Product E7889, EDTA disodium salt, exhibit?

    1 answer
    1. EDTA is a metal ion-dependent enzyme inhibitor. It may be used as a metalloprotease inhibitor at a concentration of 1-10 μm, or as a DNAse I inhibitor at a concentration of 1-10 mM.

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