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B3426

Bile salts mixture

suitable for microbiology

Synonim(y):

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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Gabaryty przesyłkiSKUDostępnośćCena netto
100 g
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1400,00 zł

Informacje o tej pozycji

NACRES:
NA.74
UNSPSC Code:
41106212

1400,00 zł


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sterility

non-sterile

Quality Level

form

powder

packaging

pkg of 100 g

loss

≤6% loss on drying

color

yellow to brownish-yellow

pH

7±0.5

solubility

distilled water: freely soluble

application(s)

microbiology

storage temp.

10-30°C

General description

Bile salts mixture is a combination of an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Bile salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic, and lithocholic acids. Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that inhibits Gram-positive bacteria and induced germination. It also inhibits the outgrowth of the germinated spores but does not interfere with the growth of Enterobacteriaceae.

Application

Bile salts mixture is recommended in diverse media like MacConkey Agar, SS Agar, and Violet Red Bile Agar. It increases the selectivity of the media to Enterobacteriaceae by inhibiting Gram-positive bacteria. It is used as a selective agent in culture media for the selective growth of intestinal bacteria like E. coli, other fecal coliforms, and fecal Streptococci. But it is also used as a selective agent in media for the detection of Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.

Preparation Note

Extracted from purified, fresh bile.
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Ta pozycja
B875648305B3883
form

powder

form

powder

form

powder

form

dried, unfractionated

application(s)

microbiology

application(s)

microbiology

application(s)

microbiology

application(s)

microbiology

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

solubility

distilled water: freely soluble

solubility

distilled water: freely soluble

solubility

H2O: 0.1 g/mL, clear, faintly yellow

solubility

water: soluble 50 mg/mL

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

-

pH

7±0.5

pH

-

pH

-

pH

-


hcodes

Hazard Classifications

Aquatic Chronic 3

Klasa składowania

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Produkty

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Powiązane treści


Chhatra B Kunwar et al.
Microbiology (Reading, England), 166(12), 1149-1159 (2020-11-19)
Colonization of the gastrointestinal tract by enterohaemorrhagic Escherichia coli (EHEC) is critically dependent on its ability to sense and respond to various microenvironments within the host. EHEC exposure to physiologically relevant levels of bile salts upregulates the two-component system, pmrAB
Crystal Gadishaw-Lue et al.
Infection and immunity, 89(2) (2020-11-25)
During passage through the human gastrointestinal tract, enterohemorrhagic Escherichia coli (EHEC) is exposed to membrane-damaging bile in the small intestine. We previously reported that EHEC treatment with a physiological bile salt mixture upregulates basRS, encoding a two-component system, and arnBCADTEF



Numer pozycji handlu globalnego

SKUNUMER GTIN
B3426-500G04061833661734
B3426-100G04061833427903

Questions

  1. What us the bile salt composition of this mixture? Is this constant between batches?

    1 answer
    1. This product is pooled from a bovine source. The bile salt mixture is composed of approximately 50% each of sodium deoxycholate and sodium cholate. The exact composition is not tested. The manufacturing process is consistent, however, given the nature of the source, some variability between lots is expected. The degree of variability does not negatively impact microbial growth. Please see section 3 of the Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sial/b3426

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