This product contains lithium dodecyl sulfate with pH at 8.4, but does not include a reducing agent. Either Dithiothrietol (DDT) or beta-mercaptoethanol (BME) may be used. The recommended final concentration is 100 mM DTT or 25 mM BME. Please see the link below to access the mPAGE™ Bis-Tris Precast SDS-PAGE Gels User Guide, page 7:_x000D_
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/384/513/20344106w-ms.pdf
MPSB-10ML
mPAGE® 4X LDS Sample Buffer
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About This Item
NACRES:
NA.56
UNSPSC Code:
41105323
$18.60
Check Cart for Availability
form
liquid
packaging
vial of 10 mL
General description
Used to prepare protein samples for denaturing polyacrylamide gel electrophoresis (PAGE) with mPAGE and most other Bis-Tris gels. LDS sample buffer contains lithium dodecyl sulfate with pH at 8.4 for optimal activity of the reducing agent.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Application
Western Blotting
Legal Information
MPAGE is a registered trademark of Merck KGaA, Darmstadt, Germany
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| form liquid | form liquid | form solution | form - |
| packaging vial of 10 mL | packaging vial of 250 mL | packaging - | packaging pkg of 1 pack |
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Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 3
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What is the composition of the loading buffer. Does it contain any reducing agents?
1 answer-
Helpful?
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Can Laemmli buffer be used instead of the sample buffer?
1 answer-
Laemmli buffer is commonly used with Tris-Glycine gels, while mPAGE gels use a different chemistry (Bis-Tris) and require LDS buffer. Therefore, it is recommended to use the LDS buffer, which can be prepared using the formula provided in the mPAGE user guide.
Helpful?
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Est ce que ce tampon contient déjà un agent réducteur tel que le DTT ou le béta mercapto ethanol ?
1 answer-
This product requires the addition of a reducing agent. Either Dithiothrietol (DDT) or beta-mercaptoethanol (BME) may be used. The recommended final concentration is 100 mM DTT or 25 mM BME. Please see the link below to access the mPAGE™ Bis-Tris Precast SDS-PAGE Gels User Guide, page 7:
https://www.emdmillipore.com/US/en/product/mPAGE-8-160-0-Bis-Tris-Precast-Gel-10x8-cm-12-well,MM_NF-MP81G12#documentationHelpful?
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