MAB3584

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

ascites fluid, clone 2HP-1H5, Chemicon^®

• Synonim(y): HP1a, Antigen p25, Chromobox Protein Homolog 5
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2HP-1H5, monoclonal
• Application: ELISA, ICC, IHC
• Citations: 41

Właściwości

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2HP-1H5, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_012117.1
• UniProt accession no.: P45973
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CBX5(23468)

Opis

General description

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Immunogen

Recombinant mouse HP1a.

Application

Immunocytochemistry: 1:500-1:5,000

Immunohistochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Western blot: not recommended. For Western blot use MAB3446.

Optimal working dilutions must be determined by the end user.

Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.

Biochem/physiol Actions

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Physical form

Liquid ascites no preservative.

Analysis Note

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable