MAB3238
Anti-Cytokeratin 19 Antibody, clone RCK108
clone RCK108, Chemicon®, from mouse
Synonim(y):
Anti-CK19, Anti-K19, Anti-K1CS
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
RCK108, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... KRT19(3880)
Opis ogólny
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8, The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases.
It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.
Specyficzność
Cytokeratin 19. MAB3238 reacts with glandular epithelial cells and most carcinomas. Does not react with hepatocytes and hepatocarcinoma
Immunogen
Cytoskeletal preparation of T24 cells.
Zastosowanie
Anti-Cytokeratin 19 Antibody, clone RCK108 is an antibody against Cytokeratin 19 for use in FC, WB, IC, IH(P).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytokeratins
Cytokeratins
Western blot: 1:100-1:1,000; Recognizes a 40kDa band in reduced westerns
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections.
Immunocytochemistry: 1:100-1:200
Flow cytometry: 1:100-1:200.
Optimal working dilutions must be determined by the end user.
Postać fizyczna
Format: Purified
Liquid in buffer with 0.1% sodium azide.
Przechowywanie i stabilność
Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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BMJ Open Gastroenterology null
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Testing null
Luca Pontiggia et al.
The Journal of investigative dermatology, 134(6), 1735-1742 (2014-01-23)
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