MAB2035

Anti-Aggrecan Antibody

CHEMICON^®, mouse monoclonal, MK-302

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: MK-302, monoclonal
• Species reactivity: canine, sheep, rat, rabbit, mouse, bovine, human
• Application: ELISA, RIA, WB
• Citations: 26

Właściwości

• Nazwa produktu: Anti-Chondroitin 6 Sulfate Antibody, clone MK-302, ascites fluid, clone MK-302, Chemicon^®
• biological source: mouse
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: MK-302, monoclonal
• species reactivity: canine, sheep, rat, rabbit, mouse, bovine, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, radioimmunoassay: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001135.2, NM_013227.2
• UniProt accession no.: P16112
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ACAN(176)

Opis

Immunogen

Chondroitinase ABC-digested adult human aggrecan.

Application

Anti-Chondroitin 6 Sulfate Antibody, clone MK-302 is an antibody against Chondroitin 6 Sulfate for use in ELISA, RIA & WB.

ELISA at 1:100-1:5,000

RIA at 1:100-1:200.

Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.

Physical form

Ascites liquid; no preservative.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable